Singlet oxygen release due to different concentrations of photosensitizer

Photodynamic therapy is a technique that consists of activating Photosensitizing Agent (PS) to form reactive oxygen species, which are important for antimicrobial action. This research aimed carry out laboratory tests measure singlet oxygenand superoxide radicals release as function different formulations and concentrations methylene blue (MB), eosin Y (EY) fluorescein (FL) dyes, compare their photodynamic efficiency. Solutions containing these compounds in MIX solvent (glycerol, ethanol water) irradiated with low power laser (λ= 660 nm) were analyzed. The production (1O2) was photometrically evaluated through the consumption rate 1-3 diphenylisobenzofuran (DPBF), 1O2 sequestering substance. Statistical analyses applied ANOVA Duncan's complementary test using Package Social Sciences (SPSS) software. MB had greater activity, it presented higher values decrease DPBF three evaluated. mean concentration 89.9% at 150 µM, 87.6% 2.9 15 77.3% 2.5 1.5 µM. EY FL similar results, no significant difference between concentrations. µM expressed 1O2, suggests effect highlights its relevancecompared other dyes.